Journal: Cancer Gene Therapy

Article Title: Long noncoding RNA LINC01291 promotes the aggressive properties of melanoma by functioning as a competing endogenous RNA for microRNA-625-5p and subsequently increasing IGF-1R expression

doi: 10.1038/s41417-021-00313-9

Figure Lengend Snippet: A LINC01291 level in melanoma was analyzed using The Cancer Genome Atlas (TCGA) database. ** P < 0.01 vs. group “Normal”. B LINC01291 expression in 41 pairs of human melanoma tissues and corresponding adjacent normal tissues was measured by quantitative real-time polymerase chain reaction (qRT-PCR). ** P < 0.01 vs. group “Normal”. C – E TCGA database was used to analyze the association between LINC01291 expression and tumor stage, overall survival, and disease-free survival in melanoma cells. F qRT-PCR analysis was used to measure LINC01291 expression in four melanoma cell lines (A-375, HT-144, SK-MEL-1, and A2058), with human epidermal melanocytes (HEMs) as a control. ** P < 0.01 vs. group “HEMs”. # P < 0.05 vs. cell lines SK-MEL-1 and A2058. G A-375 and HT-144 cells were transfected with si-LINC01291 or non-targeted siRNA and the relative expression of LINC01291 was measured by qRT-PCR. ** P < 0.01 vs. group “si-NC”. H , I Cell proliferation and colony formation abilities were determined using the Cell Counting Kit-8 and colony formation assays in A-375 and HT-144 cells after LINC01291 downregulation. ** P < 0.01 vs. “si-NC”. J , K Flow cytometry was used to analyze cell apoptosis and cell cycle status of A-375 and HT-144 cells following transfection with si-LINC01291 or non-targeted siRNA. **P < 0.01 vs. group “si-NC”. L , M Cell migration and invasion assays revealed the influence of si-LINC01291 transfection on the migration and invasion of A-375 and HT-144 cells. ** P < 0.01 vs. group “si-NC”.

Article Snippet: Human epidermal melanocytes (HEMs) were purchased from ScienCell Research Laboratories, Inc. (San Diego, CA, USA) and maintained in melanocyte medium (ScienCell Research Laboratories, Inc.).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Transfection, Cell Counting, Flow Cytometry, Migration

Journal: Frontiers in Pharmacology

Article Title: FGIN-1-27 Inhibits Melanogenesis by Regulating Protein Kinase A/cAMP-Responsive Element-Binding, Protein Kinase C-β, and Mitogen-Activated Protein Kinase Pathways

doi: 10.3389/fphar.2020.602889

Figure Lengend Snippet: Effect of FGIN-1-27 (FGIN) on melanogenesis in human melanocytes. (A) Human melanocytes were treated with FGIN-1-27 for 48 h and melanin contents were measured as described in methods. (B) Human melanocytes were treated with FGIN-1-27 for 48 h and the expression of tyrosinase, TRP-1 and TRP-2 were measured using western-blot as described in methods. (C) Human melanocytes were treated with α-MSH (50 nM) in the presence or absence of FGIN-1-27 for different times and western blot was then applied to detect MITF protein levels. (D) Human melanocytes were treated with FGIN-1-27 for the indicated time period (0–120 min), and the expression of PKC-β, p-PKA cat, PKA cat, p-CREB, CREB, p-p38, p38, p-ERK and ERK were measured by western blot. Data are expressed as the mean ± SD ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001 vs. non-treated cells.

Article Snippet: Human epidermis melanocytes (HEM) from ScienCell Research Laboratories (CA, USA) is isolated from neonatal human skin.

Techniques: Expressing, Western Blot

Journal: Frontiers in Pharmacology

Article Title: FGIN-1-27 Inhibits Melanogenesis by Regulating Protein Kinase A/cAMP-Responsive Element-Binding, Protein Kinase C-β, and Mitogen-Activated Protein Kinase Pathways

doi: 10.3389/fphar.2020.602889

Figure Lengend Snippet: Effect of FGIN-1-27 on pigmentation in guinea-pig skin. (A) Representative photographs of dorsal skin of guinea pigs. (B) The degree of depigmentation was determined by a chromameter (CR-300; Minolta, Osaka, Japan) once a week for 4 weeks. The Δ L value was calculated using the L value (brightness index) measured with the chromameter follows: Δ L = L (at each week measured) − L (at day 0). Negative Δ L values indicate an UV-induced darkening of the skin. An increase in the Δ L value indicates a decrease in hyperpigmentation induced by UV. (C) Masson–Fontana ammoniacal silver staining of skin biopsies. (D) Immunohistochemical staining of skin biopsies for the detection of S-100 as a melanocyte marker protein (E) Number of melanocytes per microscopic field in skin sections. Bar = 50 μm * p < 0.05 vs. vehicle-treated groups.

Article Snippet: Human epidermis melanocytes (HEM) from ScienCell Research Laboratories (CA, USA) is isolated from neonatal human skin.

Techniques: Silver Staining, Immunohistochemical staining, Staining, Marker